Description
Fluorescence microscopy is ideal for intracellular ion measurements and the most common of these measurements is intracellular calcium measurements. Two of the most popular dyes for calcium imaging are Fura-2 and Indo-1.
PTI EasyRatioPro is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. More importantly, PTI EasyRatioPro is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added reagents.
PTI EasyRatioPro incorporates CCD, sCMOS or EMCCD cameras for sensitive and rapid acquisition of fluorescence images from up to 16 different excitation wavelengths and optionally, four emission wavelengths. (Over 100 cameras are supported, with more added routinely.) Excitation is via our patented DeltaRam™ X compact rapid switching monochromator coupled by a liquid light guide to an upright or inverted fluorescence microscope. This allows virtually infinite excitation wavelengths from 250 to 650 nm, and acquisition rates of up to 250 for dual excitation measurements or 1000 images per second for dual emission.
Another exclusive innovation of PTI EasyRatioPro is our patented “Warp Drive” that revolutionizes imaging by increasing productivity through its innovative tactile control surface. Warp drive features real-time control of up to 60 programmable function keys and touch sensitive sliders to control exposure time, binning, gain, and wavelength selection of up to 16 different channels. It’s never been easier to optimize your images in real time!